Am J Blood Res 2012;2(2):128-135
Original Article
Time and temperature stability of T-cell subsets evaluated by a dual-platform
method
Horatiu Olteanu, Bernard C Schur, Alexandra M Harrington, Steven H Kroft
Department of Pathology, Medical College of Wisconsin, Milwaukee, WI; Dynacare Laboratories, Milwaukee, WI, USA
Received April 19, 2012; accepted May 17, 2012; Epub May 25, 2012; Published June 15, 2012
Abstract: Introduction: T-cell subset enumeration in HIV patients is routinely performed for monitoring infection stage and
response to antiretroviral therapy. Studies have examined the effect of specimen refrigeration and age for single-platform (SP)
methods, but there is limited data for time and temperature requirements of dual-platform (DP) methods. Methods: Using a
DP method, we analyzed peripheral blood (PB) from 52 HIV patients at room temperature (RT) at 24, 72, and 96 hours. PBs
from 34 HIV patients had baseline RT analysis within 24 hours, and then were refrigerated and analyzed at 24, 48, and 72
hours. The coefficient of variation (CV) and residuals (changes in lymphocyte subsets) were recorded at each time point and
compared to assess the precision and bias under the various conditions. Testing performance under different conditions was
compared by linear regression. Results: Mean CV was ≤7.3% and median residuals were <30/μl for absolute CD4 and CD8
determinations. There was good correlation between baseline analysis data at RT and at various time points, both at RT and
4°C. Conclusions: Our results are similar to those published for SP methods for aging or refrigerated specimens. The high
level of agreement between measurements supports the robustness of this DP methodology. (AJBR1204003)
Keywords: HIV, Absolute CD4 counts, flow cytometry, dual platform, specimen stability
Address all correspondence to:
Dr. Horatiu Olteanu
Department of Pathology
Medical College of Wisconsin
8701 Watertown Plank Road
Milwaukee, WI 53226, USA.
Tel: 414-805-4562; Fax: 414-805-8735
E-mail: holteanu@mcw.edu
Original Article
Time and temperature stability of T-cell subsets evaluated by a dual-platform
method
Horatiu Olteanu, Bernard C Schur, Alexandra M Harrington, Steven H Kroft
Department of Pathology, Medical College of Wisconsin, Milwaukee, WI; Dynacare Laboratories, Milwaukee, WI, USA
Received April 19, 2012; accepted May 17, 2012; Epub May 25, 2012; Published June 15, 2012
Abstract: Introduction: T-cell subset enumeration in HIV patients is routinely performed for monitoring infection stage and
response to antiretroviral therapy. Studies have examined the effect of specimen refrigeration and age for single-platform (SP)
methods, but there is limited data for time and temperature requirements of dual-platform (DP) methods. Methods: Using a
DP method, we analyzed peripheral blood (PB) from 52 HIV patients at room temperature (RT) at 24, 72, and 96 hours. PBs
from 34 HIV patients had baseline RT analysis within 24 hours, and then were refrigerated and analyzed at 24, 48, and 72
hours. The coefficient of variation (CV) and residuals (changes in lymphocyte subsets) were recorded at each time point and
compared to assess the precision and bias under the various conditions. Testing performance under different conditions was
compared by linear regression. Results: Mean CV was ≤7.3% and median residuals were <30/μl for absolute CD4 and CD8
determinations. There was good correlation between baseline analysis data at RT and at various time points, both at RT and
4°C. Conclusions: Our results are similar to those published for SP methods for aging or refrigerated specimens. The high
level of agreement between measurements supports the robustness of this DP methodology. (AJBR1204003)
Keywords: HIV, Absolute CD4 counts, flow cytometry, dual platform, specimen stability
Address all correspondence to:
Dr. Horatiu Olteanu
Department of Pathology
Medical College of Wisconsin
8701 Watertown Plank Road
Milwaukee, WI 53226, USA.
Tel: 414-805-4562; Fax: 414-805-8735
E-mail: holteanu@mcw.edu
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