Am J Blood Res 2011;1(2):167-174
Original Article
Use of IGK gene rearrangement analysis for clonality assessment of lymphoid
malignancies. A single center experience
Claudia Mannu, Anna Gazzola, Francesco Bacci, Elena Sabattini, Carlo Sagramoso, Fernando Roncolato, Maura Rossi, Maria
Antonella Laginestra, Maria Rosaria Sapienza, Claudio Agostinelli, Antonio De Leo, Milena Piccioli, Simona Righi, Patrizia
Artioli, Luigi Chilli, Gianpaolo Da Pozzo, Giuseppe De Biase, Federica Sandri, Stefano A Pileri, Pier Paolo Piccaluga
Hematopathology Section, Department of Hematology and Oncological Sciences “L. and A. Seràgnoli”, S. Orsola-Malpighi
Hospital, University of Bologna, Italy; Department of Haematology, St George Hospital, Sydney, Australia.
Received August 9, 2011; accepted August 27, 2011; Epub September 12, 2011; published September 30, 2011
Abstract: Background: Anemia is an important cause of morbidity in MDS patients, principally through increased
cardiovascular disease. Transfusion status has been seen to be a significant prognostic factor for disease progression and
mortality, yet the relationship between anemia levels and cardiovascular disease is not well understood. Objective: This study
aimed to review the published literature on the effect of anemia on cardiovascular outcomes in patients with MDS. Methods: A
systematic literature review was undertaken to identify studies that investigated the relationship between anemia (as defined
by hemoglobin levels) and cardiovascular outcomes in patients with MDS (all subtypes). Results: Three studies were
identified that explicitly evaluated the relationship between anemia and cardiovascular outcomes in MDS, and another study
reported the relationship between hemoglobin levels and survival. The four studies consistently showed a strong relationship
between lower hemoglobin levels and worse cardiovascular outcomes, including cardiac remodeling, congestive heart
failure, coronary artery disease, myocardial infarction, arrhythmia, heart valve disease, and cardiovascular mortality. Anemia
was seen to be an independent predictor of cardiovascular disease outcomes in patients with MDS, beyond transfusion
status and IPSS. Conclusion: Based upon a relatively small body of evidence, there appears to be a strong and clinically
significant association between anemia and cardiovascular morbidity and mortality in MDS. While further research is needed,
clinicians should seek to actively manage hemoglobin levels in MDS patients before the point of transfusion dependency is
reached. (AJBR1108002).
Keywords: BIOMED-2, molecular diagnostic, IGK, non Hodgkin lymphoma, PCR
Full Text PDF
Address all correspondence to:
Pier Paolo Piccaluga, MD, PhD
Molecular Pathology Laboratory,
Haematopathology Unit, Department of Hematology and Oncology "L. and A. Seràgnoli",
S. Orsola-Malpighi Hospital, University of Bologna
Via Massarenti, 9 - 40138 Bologna, Italy.
Phone: 0039-051-6364043; Fax: 0039-051-6364037;
e-mail: pierpaolo.piccaluga@unibo.it
Original Article
Use of IGK gene rearrangement analysis for clonality assessment of lymphoid
malignancies. A single center experience
Claudia Mannu, Anna Gazzola, Francesco Bacci, Elena Sabattini, Carlo Sagramoso, Fernando Roncolato, Maura Rossi, Maria
Antonella Laginestra, Maria Rosaria Sapienza, Claudio Agostinelli, Antonio De Leo, Milena Piccioli, Simona Righi, Patrizia
Artioli, Luigi Chilli, Gianpaolo Da Pozzo, Giuseppe De Biase, Federica Sandri, Stefano A Pileri, Pier Paolo Piccaluga
Hematopathology Section, Department of Hematology and Oncological Sciences “L. and A. Seràgnoli”, S. Orsola-Malpighi
Hospital, University of Bologna, Italy; Department of Haematology, St George Hospital, Sydney, Australia.
Received August 9, 2011; accepted August 27, 2011; Epub September 12, 2011; published September 30, 2011
Abstract: Background: Anemia is an important cause of morbidity in MDS patients, principally through increased
cardiovascular disease. Transfusion status has been seen to be a significant prognostic factor for disease progression and
mortality, yet the relationship between anemia levels and cardiovascular disease is not well understood. Objective: This study
aimed to review the published literature on the effect of anemia on cardiovascular outcomes in patients with MDS. Methods: A
systematic literature review was undertaken to identify studies that investigated the relationship between anemia (as defined
by hemoglobin levels) and cardiovascular outcomes in patients with MDS (all subtypes). Results: Three studies were
identified that explicitly evaluated the relationship between anemia and cardiovascular outcomes in MDS, and another study
reported the relationship between hemoglobin levels and survival. The four studies consistently showed a strong relationship
between lower hemoglobin levels and worse cardiovascular outcomes, including cardiac remodeling, congestive heart
failure, coronary artery disease, myocardial infarction, arrhythmia, heart valve disease, and cardiovascular mortality. Anemia
was seen to be an independent predictor of cardiovascular disease outcomes in patients with MDS, beyond transfusion
status and IPSS. Conclusion: Based upon a relatively small body of evidence, there appears to be a strong and clinically
significant association between anemia and cardiovascular morbidity and mortality in MDS. While further research is needed,
clinicians should seek to actively manage hemoglobin levels in MDS patients before the point of transfusion dependency is
reached. (AJBR1108002).
Keywords: BIOMED-2, molecular diagnostic, IGK, non Hodgkin lymphoma, PCR
Full Text PDF
Address all correspondence to:
Pier Paolo Piccaluga, MD, PhD
Molecular Pathology Laboratory,
Haematopathology Unit, Department of Hematology and Oncology "L. and A. Seràgnoli",
S. Orsola-Malpighi Hospital, University of Bologna
Via Massarenti, 9 - 40138 Bologna, Italy.
Phone: 0039-051-6364043; Fax: 0039-051-6364037;
e-mail: pierpaolo.piccaluga@unibo.it
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